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Segments are flanked by conserved sequences called recombination signal sequences (RSS), comprised a heptameric and a nonameric sequence, which are interposed by either a 12 (RSS-12) or a 23 (RSS-23) base pair sequence. VDJ rearrangement is brought about by recombinase-activating gene-1 (RAG-1) and RAG-2 protein complexes, which always bring together segments with a RSS-12 with a segment flanked b
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